Molecular, genetic, physiological and biochemical studies of 3-methylcrotonyl-CoA carboxylase and biotin carboxyl carrier protein-like proteins in Arabidopsis thaliana
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چکیده
3-Methylcrotonyl-CoA carboxylase (MCCase) is a nuclear-encoded, mitochondriallocalized biotin-containing enzyme. The reaction catalyzed by this enzyme is required for leucine (Leu) catabolism in mitochondria, and it may also play a role in the catabolism of isoprenoids and in the mevalonate shunt. In Arabidopsis, two MCCase subunits (the biotinylated MCCA subunit and the non-biotinylated MCCB subunit) are each encoded by single genes (At1g03090 and At4g34030, respectively). A reverse genetic approach was undertaken to dissect the physiological role of MCCase in plants. We have recovered and characterized T-DNA and transposon knockout alleles of MCCA and MCCB genes.
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تاریخ انتشار 2016